Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

CHEN Xiao-Ming; TAN Bi-Sheng; ZHENG Chun; ZHANG Jian-Guo; WANG Dan; CHU Shi-Jin; REN Zheng-Long

Chinese Physics C> 2007, Vol. 31> Issue(10) : 972-977

Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

Southwest University of Science and Technology, Mianyang 621010, China2 Sichuan Agriculture University, Yaan 625000, China3 Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

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Abstract：
The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

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CHEN Xiao-Ming, TAN Bi-Sheng, ZHENG Chun, ZHANG Jian-Guo, WANG Dan, CHU Shi-Jin and REN Zheng-Long. Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation[J]. Chinese Physics C, 2007, 31(10): 972-977.

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Received: 2006-10-19
Revised: 2007-03-22

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

Corresponding author: REN Zheng-Long,

Southwest University of Science and Technology, Mianyang 621010, China2 Sichuan Agriculture University, Yaan 625000, China3 Institute of Nuclear Physics and Chemistry, China Academy of Engineering Physics, Mianyang 621900, China

Received Date: 2006-10-19

Accepted Date: 2007-03-22

Available Online: 2007-10-05

Abstract: The content of Bacillus substilis var. niger (ATCC 9372) DNA double-strand breaks induced by neutron radiation at different doses and different dose rates respectively, was measured and quantified by pulsed-field gel electrophoresis. By the analyses of DNA release percentage (PR), DNA breakage level (L), average molecular weight of breakage DNA and DNA fragment distribution, the results showed that DNA fragments were obviously distributed into two regions in the different irradiation condition. This suggestes that there are some sensitive points to neutron radiation in the Bacillus substilis var. niger molecule. The DNA release percentage, DNA breakage level and the content of DNA fragments in different region varied regularly in some extent with change of the neutron radiation dose or dose rate.

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Studies on Bacillus substilis DNA Lesions Induced by Fast Neutron Radiation

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