Impact factor of Chinese Physics C is 3.6 in 2023 Impact factor of Chinese Physics C is 3.6 in 2022 2021 Impact Factor 2.944
Advanced Search
Chinese Physics C> 2003, Vol. 27> Issue(S1) : 19-22 

XAS Study of a Venom Metalloprotein

  •  Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China

  • In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.
  • 加载中

  • References

    [1] Hasnain S S, Hodgson K 0. J. Synchrolron Rad. , 1999, 6 : 8522 Cranier S P. X-Ray Ahsorplion : Principles, Applicalions , Techniques of EXAFS, SEXAFS and XANES. Koningsherger D C and Prins R, eds, New York: Wiley , 1988. 2573 Scott R A. Slructural and Resonance Techniques in Biological Research.( D. L. Rousseau, ed. ) , Orlando, Florida: Academic Press, 1984. 2954 Powers L. Biochini. Biophys. Acta, 1982, 1 :6835 Rehr J J, Ankudinov A L. J. Synchrotron Rad. , 2003 , 10: 436 WU Z Y et al. Physica Scripla, 2003 (in press)7 Naloli C R, Benfatto M, Longa S D el al. J. Synchrotron Rad. ,2003, 10 : 268 Hite L A , Shannon J D, Rjarnason J B et al. Biochemistry, 1992, 31(27 ) : 62039 Hite L A , Jia I. G. , Bjarnason J B et al. Arch. Biochem. Biophys. ,1994, 308(1): 18210 GONG W, ZHU X , LIU S et al. J. Mol. Biol. , 1998, 283 ( 3 ) :65711 XU X, WANG C, LIU J et al. Toxicon, 1981 , 19(5) : 63312 Hasnain S S, Strange R W. J. Synchrotron Rad. , 2003, 10:9

  • Access

    加载中

Get Citation
LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22.
LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22. shu
Milestone
Received: 2003-11-21
Revised: 1900-01-01
Article Metric

Article Views(3905)
PDF Downloads(605)
Cited by(0)
Policy on re-use
To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Email This Article

Title:
Email:

XAS Study of a Venom Metalloprotein

    Corresponding author: LI Shu-Jun,
  • Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China
  • Received Date: 2003-11-21
  • Accepted Date: 1900-01-01
  • Available Online: 2003-01-02

Abstract: In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.

    HTML

Reference (1)

目录

Link
IOPScience
SCOAP3
arXiv
Chinese Physical Society
Journal information
Introduction
Editorial Board
Others
Subscribe
Contact us
QQ: 1307685413
Tel: +86-010-88235947
Fax: +86-010-88233126
E-mail: cpc@ihep.ac.cn

  • CPC Website

  • IOP Website

  • CPC WeChat

Copyright © Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Road, Beijing 100049, China 京ICP备05002789号-1

Supported by: Beijing Renhe Information Technology Co. Ltd  E-mail: info@rhhz.net

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return