XAS Study of a Venom Metalloprotein

LI Shu-Jun; ZHAO Wei; CHU Wang-Sheng; NIU Li-Wen; TENG Mai-Kun; HU Tian-Dou; XIE Ya-Ning; WU Zi-Yu

Chinese Physics C> 2003, Vol. 27> Issue(S1) : 19-22

XAS Study of a Venom Metalloprotein

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China

Abstract
HTML
Reference
Related

PDF

Abstract：
In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.

References

[1]

Hasnain S S, Hodgson K 0. J. Synchrolron Rad. , 1999, 6 : 8522 Cranier S P. X-Ray Ahsorplion : Principles, Applicalions , Techniques of EXAFS, SEXAFS and XANES. Koningsherger D C and Prins R, eds, New York: Wiley , 1988. 2573 Scott R A. Slructural and Resonance Techniques in Biological Research.( D. L. Rousseau, ed. ) , Orlando, Florida: Academic Press, 1984. 2954 Powers L. Biochini. Biophys. Acta, 1982, 1 :6835 Rehr J J, Ankudinov A L. J. Synchrotron Rad. , 2003 , 10: 436 WU Z Y et al. Physica Scripla, 2003 (in press)7 Naloli C R, Benfatto M, Longa S D el al. J. Synchrotron Rad. ,2003, 10 : 268 Hite L A , Shannon J D, Rjarnason J B et al. Biochemistry, 1992, 31(27 ) : 62039 Hite L A , Jia I. G. , Bjarnason J B et al. Arch. Biochem. Biophys. ,1994, 308(1): 18210 GONG W, ZHU X , LIU S et al. J. Mol. Biol. , 1998, 283 ( 3 ) :65711 XU X, WANG C, LIU J et al. Toxicon, 1981 , 19(5) : 63312 Hasnain S S, Strange R W. J. Synchrotron Rad. , 2003, 10:9

[1]	LIU Ying-Bin , GAO Yu-Xi , CHEN Chun-Ying , LI Bai , YU Hong-Wei , WANG Jiang-Xue , HE Wei , HUANG Yu-Ying , CHAI Zhi-Fang . Quantitative Analysis of Iron in Protein Bands Existing in Polyacrylamide Gel Matrix with Synchrotron Radiation. Chinese Physics C, 2005, 29(S1): 61-64.
[2]	WANG Fa-Ya . Design of Accelerating Structure on a Novel Compact Electron Linac. Chinese Physics C, 2004, 28(10): 1109-1115.
[3]	HU Zheng-Guo , GUO Zhong-Yan , ZHAN Wen-Long , XIAO Guo-Qing , XU Hu-Shan , SUN Zhi-Yu , WANG Meng , MAO Rui-Shi , CHEN Zhi-Qiang , CHEN Li-Xin , LI Chen , BAI Jie , ZHANG Jin-Xia , LI Cun-Pan . A Multi-Anode Transverse Field Gas lonization Chamber. Chinese Physics C, 2003, 27(10): 905-908.
[4]	HONG Xin-Guo , WENG Yu-Xiang , LI Ming . Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering. Chinese Physics C, 2003, 27(S1): 57-62.
[5]	CHU Wang-Sheng , HU Tian-Dou , XIE Ya-Ning , LIU Wen-Han , WU Zi-Yu , . Metal K-edge XAS Study of AlB2-type Transition-metal Diborides. Chinese Physics C, 2003, 27(S1): 10-14.
[6]	HUANG Sheng-Li , XU Zi-Zong , WANG Zhao-Min , WANG Xiao-Lian , LI Cheng , CHEN Hong-Fang , WU Jian , ZENG Hui . A Precise Trigger Timing System. Chinese Physics C, 2002, 26(9): 959-963.
[7]	SHENG Yi , LI Xiao-Wei , LI Dong-Mei , TAO Ye . Study on Vacuum ltraviolet Circular Dichroism Spectra of Several Proteins. Chinese Physics C, 2001, 25(S1): 66-69.
[8]	YAN WenBiao , YANG ChunBin , CAI Xu . A Poissible Method to Determine the Order of QGP Phase Transitions. Chinese Physics C, 2000, 24(9): 852-857.
[9]	Ding Linkal , Zhu Qingqi , Shen Changquan , Yao Zhiguo , Cheung Tsang , Matti Vallinkoski . An Approach to Study the Cosmic Ray ‘Knee’Composition From Underground Multimuon Events at a Shallower Depth. Chinese Physics C, 1999, 23(6): 584-588.
[10]	Wang Yunyong , Wang Shaomin , Mao Huishun , Liu Rongguang , Li Rubai , Li Jin , Song Xiaofei , Zhang Jian , Zhang Dehong , Jin Yan , Huang Jundong , Ke Zunjian , Gao Shuqi , Xie Peipei , Yan Jie , Gu Jianhui , Li De . Study of the Unusual Performances of One Type DriftChamber Feedthrough. Chinese Physics C, 1998, 22(5): 398-405.
[11]	Wu Chongshi . An Improved Schematic Pairing Force. Chinese Physics C, 1997, 21(1): 62-66.
[12]	NI Guang-Jiong , ZHANG Min , GONG Jia-Wen . A Rigorous Method for Calculating the Casimir Effect. Chinese Physics C, 1991, 15(8): 695-700.
[13]	YU Hong . A NEW METHOD FOR THE DETERMINATION OF THE SPIN OF ξ(2230). Chinese Physics C, 1989, 13(1): 87-91.
[14]	GAO Xiao-Chun , WANG Yong . THE STUDY OF A SOLITON SOLUTION IN THE SKYRME MODEL WITH B=2. Chinese Physics C, 1988, 12(3): 341-350.
[15]	XU Jian-Ming , XU Qing . A NEW PROJECT OF MULTIPOLE PERMANENT MAGNETS. Chinese Physics C, 1986, 10(1): 120-123.
[16]	LONG Ming , HUANG Tao . A POSSIBLE FORM OF THE PION'S STRUCTURE FUNCTION. Chinese Physics C, 1986, 10(5): 562-569.
[17]	LI Jin , JIANG Chun-Hua . A NEW CYLINDRICAL LIGHT GUIDE. Chinese Physics C, 1984, 8(1): 122-124.
[18]	WANG YUN-YONG . A NEW METHOD TO MEASURE THE WIRE TENSION. Chinese Physics C, 1982, 6(6): 771-774.
[19]	Wu Bang-hui , Liu Yong-xian , Hu Shi-ke . A POSSIBLE TYPE OF POTENTIAL FOR THE BOUND STATE OF MESONS. Chinese Physics C, 1980, 4(5): 665-670.
[20]	ZHOU LI-NONG , XIE JIA-LIN . A BI-PERIODIC ACCELERATOR STRUCTURE. Chinese Physics C, 1979, 3(2): 165-175.

Access

Get Citation

LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22.

LI Shu-Jun, ZHAO Wei, CHU Wang-Sheng, NIU Li-Wen, TENG Mai-Kun, HU Tian-Dou, XIE Ya-Ning and WU Zi-Yu. XAS Study of a Venom Metalloprotein[J]. Chinese Physics C, 2003, 27(S1): 19-22. shu

RIS(for EndNote,Reference Manager,ProCite)

BibTex

Txt

Milestone

Received: 2003-11-21
Revised: 1900-01-01

Article Metric

Article Views(3103)
PDF Downloads(605)
Cited by(0)

Policy on re-use

To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

XAS Study of a Venom Metalloprotein

Corresponding author: LI Shu-Jun,

Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 1000392 University of Science and Technology of China,Hefei 230026,China

Received Date: 2003-11-21

Accepted Date: 1900-01-01

Available Online: 2003-01-02

Abstract: In this paper,we probed the Acutolysin A—a Zinc protein purified from venom-by XAS technique,and its XAS curves in solution state of different pH values,were obtained.It was confirmed that the intense X-Rays didnt damage the samples during the experiment,and the curves among different pH values did present some differences.Through the comparison between simulated EXAFS curve based on PDB structure and an experimental signal,we concluded that the PDB data does not qualify for the fine structure research due to the limited resolution.At the same time,XANES analysis result could not reconstruct the experimental EXAFS curve either,because the PDB cluster used in the XANES calculation is free of Hydrogen atoms,therefore,a Hydrogen-contained cluster might be essential to the good fitting of XANES.

HTML

Reference (1)

XAS Study of a Venom Metalloprotein

Abstract：

References

Access

Article Metrics

Metrics

通讯作者: 陈斌, bchen63@163.com

Email This Article