Investigation of DNA Strand Breaks Induced by <SUP>7</SUP>Li and <SUP>12</SUP>C Ions

SUI Li; ZHAO Kui; NI Mei-Nan; GUO Ji-Yu; LUO Hong-Bing; MEI Jun-Ping; LU Xiu-Qin; ZHOU Ping

Chinese Physics C> 2004, Vol. 28> Issue(10) : 1126-1130

Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions

China Institute of Atomic Energy,Beijing 102413,China2 The Key Laboratory of Beam Technology and Material Modification of Ministry of Education,Beijing Normal University,Beijing 100875,China3 College of Agronomy,Hunan Agricultural University,Changsha 410128,China4 Hebei University of Technology,Tianjin 300130,China

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Abstract：
Deoxyribonucleic acid (DNA) is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects. Numerous lesions have been identified in irradiated DNA. DNA double strand breaks (DSBs) are considered as the most important initial damage of all biological effects induced by ionizing radiation. In this experiment,DNA DSBs induced by heavy ions in the early period were investigated with atomic force microscopy (AFM). Choosing ⁷Li and ¹²C heavy ions with different LET values delivered by HI 13 tandem accelerator respectively,purified plasmid DNA samples in aqueous solution were irradiated at different doses. AFM was used for nanometer level structure analysis of DNA damage induced by these two kinds of heavy ions. Measurement of the DNA fragment lengths was accomplished with the Scion Image analyzed software. Change laws of three forms of DNA,supercoil,open circular and linear form as dose increased were obtained. Distributed function of DNA fragment length was also obtained,and fitted with Tsallis entropy statistical theory.

References

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SUI Li, ZHAO Kui, NI Mei-Nan, GUO Ji-Yu, LUO Hong-Bing, MEI Jun-Ping, LU Xiu-Qin and ZHOU Ping. Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions[J]. Chinese Physics C, 2004, 28(10): 1126-1130.

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Received: 2003-12-30
Revised: 1900-01-01

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

Investigation of DNA Strand Breaks Induced by ⁷Li and ¹²C Ions

Corresponding author: SUI Li,

China Institute of Atomic Energy,Beijing 102413,China2 The Key Laboratory of Beam Technology and Material Modification of Ministry of Education,Beijing Normal University,Beijing 100875,China3 College of Agronomy,Hunan Agricultural University,Changsha 410128,China4 Hebei University of Technology,Tianjin 300130,China

Received Date: 2003-12-30
Accepted Date: 1900-01-01
Available Online: 2004-10-05

Abstract: Deoxyribonucleic acid (DNA) is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects. Numerous lesions have been identified in irradiated DNA. DNA double strand breaks (DSBs) are considered as the most important initial damage of all biological effects induced by ionizing radiation. In this experiment,DNA DSBs induced by heavy ions in the early period were investigated with atomic force microscopy (AFM). Choosing ⁷Li and ¹²C heavy ions with different LET values delivered by HI 13 tandem accelerator respectively,purified plasmid DNA samples in aqueous solution were irradiated at different doses. AFM was used for nanometer level structure analysis of DNA damage induced by these two kinds of heavy ions. Measurement of the DNA fragment lengths was accomplished with the Scion Image analyzed software. Change laws of three forms of DNA,supercoil,open circular and linear form as dose increased were obtained. Distributed function of DNA fragment length was also obtained,and fitted with Tsallis entropy statistical theory.